Flow Cytometry

Flow Cytometry Shared Resource

Hours of operation

The resource will be open 24 hours/day, 7 days each week.

Staff Support

Resource staff will be available to support investigators from 9AM to 5PM, Monday through Friday. To ensure support during that time, pre-scheduling of operator support is required.  Support from 5PM to 9PM may be available for work with irreplaceable specimens (please inquire).

Recent Notices

Dr. Mike Munks joined the core as the Director of the Flow Cytometry and Monoclonal Antibody Core in October 2022.

Christina Metea joined the core full-time as an Assistant Core Scientist in July 2022.

Reshma Purohit joined the core as an Associate Core Scientist in February 2022.

We appreciate your patience as Pamela Canaday, our Senior Core Scientist, continues training new staff.

The new KCRB Cytek Aurora (Frodo) arrived in October of 2022.

The Flow Cytometry Core and Monoclonal Antibody Service has been merged into a single University Shared Resource.

The core iLab site and website are undergoing significant revisions. Email Christina Metea (meteac@ohsu.edu) and Mike Munks (munksm@ohsu.edu) if you encounter functionality problems or have suggestions.

Policies

PPE Requirements:

Many investigators using the shared resource are studying human cells and/or lentivirally transduced cells. As such, the laboratory is considered a BSL2 laboratory (with some sorts considered BSL2 with added precautions/procedures; or BSL2+); requiring PPE to keep staff and users safe from potentially infectious agents within specimens. PPE requirements for the shared resource follow:

• Masks, gloves, laboratory coats, and eye protection (glasses, goggles, or face shields) are required at all times within the laboratory.
• Gloves will be provided by the laboratory; additional PPE are the responsibility of user laboratories.
• The following glove practice must be followed: Upon entering laboratory, gloves should be put on; prior to exiting laboratory, gloves should be removed and hands washed.

Work Area and Instrument Cleaning

Resource staff will clean at the start of the work day, users will clean both before and after they use the instrumentation.  Please allow for an extra 10 minutes to your appointment for cleaning.  Methods and materials will be provided at each workstation.

Daily instrument QA/QC will be conducted each morning by resource staff.

• Pamela Canaday: canadayp@ohsu.edu
• Reshma Purohit: purohitr@ohsu.edu
• Kyle Taylor: taylorky@ohsu.edu
• Christina Metea: meteac@ohsu.edu

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Overview of Services

The OHSU Flow Cytometry Shared Resource (FCSR) has operated as a core resource for OHSU

researchers and the Knight Cancer Institute members since 1996 and the Oregon Stem Cell

Center since 2005. It provides advanced flow cytometry instrumentation, technical expertise

and technical services.

The FCSR also provides training in data interpretation, experiment design and routine

instrument operation, offering investigators a cost-saving option of doing some of the work

themselves. Finally, this resource saves valuable investigator time by analyzing specimens and

preparing them if needed.

Location

Richard Jones Hall (RJH)

Room 5380                          

Lab Phone: 503-418-2986 (Ext. 8-CYTO)         

Knight Cancer Research Building (KCRB)

Room 4161

Lab Phone: 503-494-6145          

Links and Resources

Core Website        http://www.ohsu.edu/flowcytometry

 MAP to the Flow Cytometry Shared Resource RJH

Contacts

Available Equipment and Resources