ALERT

In Observance of U.S. Holiday, Martin Luther King Jr. Day, Agilent Cross Lab/ iLab Operations Software will be closed during U.S. hours on Monday January 21st, 2019. We will resume regular U.S. support hours on Tuesday January 22nd, 2018. EU and APAC Support will remain open during this time. For urgent matters, please add "Urgent" to the ticket/ email subject or press "1", when prompted, to escalate a call on the iLab Support phone and we will prioritize those requests first.

Flow Cytometry

Announcements:

  

Available instruments at KCRB are the ARIAFusion, Fortessa, MacsQuant and Vi-Cell cell counter. We also have a new computer workstation at KCRB with FlowJo and FCS Express to aid in your flow analysis.

 

There is a new 19-color analytical instrument, the BD FACSymphony, available in RJH. Contact for the flow core staff for training.

  

 

The KCRB Aria Fusion Sorter is available with an operator EVERY DAY!

Please book:

Dorian: Monday, Wednesday, Friday

Bri: Tuesday and Thursday

Or

Pamela: Tuesday-Friday 

 

 

To book time on an instrument or with an operator please choose the "Schedule Equipment" tab above.

 

 

OHSU Research Cores and Shared Resources Acknowledgement & Authorship Policies

Acknowledgement Policy

The use of data generated in a core facility in a grant application, progress report or publication contains the implicit understanding that the PI or authors will acknowledge the use of the core facility.  Since many of our facilities are supported by federal agencies, such acknowledgments are essential for obtaining continuing support. 

Authorship Policy

If core personnel provide significant intellectual input to the results submitted for publication, then it is reasonable and appropriate to include them as co-authors.  Since circumstances vary widely, each case should be considered individually, and the core should be consulted for review prior to publishing.

 

Overview of Services

 

The OHSU Flow Cytometry Shared Resource (FCSR) has operated as a core resource for OHSU

researchers and the Knight Cancer Institute members since 1996 and the Oregon Stem Cell

Center since 2005. It provides advanced flow cytometry instrumentation, technical expertise

and technical services.

 

The FCSR also provides training in data interpretation, experiment design and routine

instrument operation, offering investigators a cost-saving option of doing some of the work

themselves. Finally, this resource saves valuable investigator time by analyzing specimens and

preparing them, if needed. For more information, please contact the core director Philip Streeter

or click this link to visit our website. 

 

Location and hours of operation

Richard Jones Hall (RJH)  5380                            |              Knight Cancer Research Building (KCRB) 4161

     Lab Phone: 503-418-2986 (Ext. 8-CYTO)          |                   Lab Phone: 503-494-6145

 

• Operators available for assistance Monday-Friday 9AM-5PM      

• Open 24/7 to trained investigators with access                         


Links and Resources

Core Website        http://www.ohsu.edu/flowcytometry


Instrument Laser and Filter Configurations

 

Protocols

          General Immunofluorescence Staining Protocol

          Cell Cycle Analysis 

          LSR setup and general use

          MACSQuant Quick Guide

          Vi-Cell Quick Guide

 

 MAP to the Flow Cytometry Shared Resource

                               

 

Contacts

Name Role Phone Email Location
Philip Streeter PhD
Core Director
 
503 494-1762
 
streetep@ohsu.edu
 
Biomedical Research Building (BRB) 7
 
Brianna Garcia
Flow Cytometry Operator
 
503 494-6145
 
garciabr@ohsu.edu
 
Knight Cancer Research Building (KCRB) 4161
 
Dorian LaTocha
Flow Cytometry Operator
 
503 494-6145
 
latochad@ohsu.edu
 
Knight Cancer Research Building (KCRB) 4161
 
Sara Christensen
Flow Cytometry Operator
 
503 494-0661
 
christes@ohsu.edu
 
Richard Jones Hall (RJH) 5379
 
Pamela Canaday
Flow Cytometry Operator
 
503 494-0661
 
canadayp@ohsu.edu
 
Richard Jones Hall (RJH) 5379
 
Matthew Lewis
Flow Cytometry Operator
 
503 494-0661
 
spooner@ohsu.edu
 
Richard Jones Hall (RJH) 5379
 

Available Equipment and Resources

Core Personnel (4)
KCRB Analyzers (2)
RJH Analyzers (6)
Sorter schedules for reference only (3)
Workstation (2)

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