Flow Cytometry

Flow Cytometry Shared Resource

Hours of Operation: Both sites are badge-accessible 24 hours per day, 7 days per week.

Staff Support: Core Scientists are available Monday to Friday, from 9 AM to 5 PM, excluding OHSU holidays. Pre-scheduling of Core Scientist time will help us meet your needs. For irreplacable samples, support from 5 PM to 9 PM may be available (please inquire).

Overview of Services

The OHSU Flow Cytometry and Monoclonal Antibody Shared Resource (FCMAbSR) provides advanced flow cytometry instrumentation and related services. The core also provides monoclonal antibody development, screening, expression and purification services. Technical assistance includes consultations, letters of support, instrument training, software training, experimental design and data interpretation. Core services are available to OHSU, the VA, PSU and the surrounding Portland community. The core has operated since 1996.

Recent Notices

2024: Abby Buenafe joined as Associate Core Scientist (January). Christina Metea was promoted to Associate Core Scientist (March). The RJH Flow Core relocated due to flooding (February), then moved back (April).

2023: Yong-Ping Zhong joined as Research Associate, and the Monoclonal Antibody Core officially merged with the Flow Core (July). Kyle Taylor resigned (December).

2022: Mike Munks became Director (November). Phil Streeter retired (October). The new Cytek Aurora (Frodo) arrived at KCRB. Kyle Taylor joined as a Core Scientist (August). Christina Metea joined as an Assistant Core Scientist 2 (July). Reshma Purohit joined the Flow Core as an Associate Core Scientist.

Policies

PPE Requirements:

Many investigators using the shared resource are studying human cells and/or lentivirally transduced cells. As such, the laboratory is considered a BSL2 laboratory (with some sorts considered BSL2 with added precautions/procedures; or BSL2+); requiring PPE to keep staff and users safe from potentially infectious agents within specimens. PPE requirements for the shared resource follow:

• Masks, gloves, laboratory coats, and eye protection (glasses, goggles, or face shields) are required at all times within the laboratory.
• Gloves will be provided by the laboratory; additional PPE are the responsibility of user laboratories.
• The following glove practice must be followed: Upon entering laboratory, gloves should be put on; prior to exiting laboratory, gloves should be removed and hands washed.

Work Area and Instrument Cleaning

Resource staff will clean at the start of the work day, users will clean both before and after they use the instrumentation.  Please allow for an extra 10 minutes to your appointment for cleaning.  Methods and materials will be provided at each workstation.

Daily instrument QA/QC will be conducted each morning by resource staff.

• Pamela Canaday: canadayp@ohsu.edu
• Reshma Purohit: purohitr@ohsu.edu
• Christina Metea: meteac@ohsu.edu
• Abby Buenafe: buenafea@ohsu.edu

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Locations

Knight Cancer Research Building: KCRB Room 4161. Lab Phone: 503-494-6145. KCRB location on GoogleMaps       

Richard Jones Hall: RJH Room 5380. Lab Phone: 503-418-2986 (Ext. 8-CYTO). RJH location on GoogleMaps.

Links & Resources
Visit the core's website for information on instrumentation, training, FlowJo and FCS Express software licenses, and for monoclonal antibody services:        http://www.ohsu.edu/flowcytometry

Questions or suggestions regarding the iLab site and core website should be directed to Christina Metea (meteac@ohsu.edu) and Mike Munks (munksm@ohsu.edu).

Contacts

Available Equipment and Resources